PIK3R1 antibody (pTyr607)

Catalog No. ABIN6284440

Product Details anti-PIK3R1 Antibody

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Binding Specificity: AA 550-630, pTyr607

Reactivity: Human, Mouse, Rat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: PI 3-kinase p85α is a protein encoded by the PIK3R1 gene which is approximately 83,5 kDa. PI 3-kinase p85α is localised to the cytoplasm, cytosol and nucleus. It is involved in RET signalling, the IL-2 pathway, downstream signalling of activated FGFR2, regulation of lipid metabolism and insulin signalling-generic cascades. It binds to activated protein-Tyr kinases, through its SH2 domain, and acts as an adapter in mediating the association of the p110 catalytic unit to the plasma membrane. It is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. PI 3-kinase p85α isoform 2 and isoform 4 are present in skeletal muscle. Mutations in the PIK3R1 gene may result in Short syndrome and Agammaglobulinemia. STJ90925 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous PI 3-kinase p85α protein which only binds about Y607 when Y607 is phosphorylated.

Specificity: Phospho-PI 3-kinase p85α (Y607) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85α protein only when phosphorylated at Y607.

Characteristics: Rabbit polyclonal to Phospho-PI 3-kinase p85α (Y607).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PI 3-kinase p85alpha around the phosphorylation site of Y607.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000

Comment:

Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for PIK3R1

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Alternative Name: PI 3-kinase p85alpha (PI3K p85a Products)

Synonyms: PI3CG antibody, PI3K antibody, PI3Kgamma antibody, PIK3 antibody, AGM7 antibody, GRB1 antibody, p85 antibody, p85-ALPHA antibody, PI3KA antibody, AA414921 antibody, C530050K14 antibody, p50alpha antibody, p55alpha antibody, p85alpha antibody, wu:fb65b05 antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma antibody, phosphoinositide-3-kinase regulatory subunit 1 antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta antibody, phosphoinositide-3-kinase, regulatory subunit 1 (alpha) antibody, PIK3CG antibody, PIK3R1 antibody, Pik3r1 antibody, Pik3cb antibody, pik3r1 antibody

Molecular Weight: 85 kDa

Gene ID: 5295

UniProt: P27986

Pathways: TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effect