Vinculin antibody (AA 760-840)

Catalog No. ABIN6285011

Product Details anti-Vinculin Antibody

Target: Vinculin (VCL)

Binding Specificity: AA 760-840

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Vinculin is a protein encoded by the VCL gene which is approximately 123,7 kDa. Vinculin is localised to the cell membrane. It is involved in RET signalling, signalling by moderate kinase activity BRAF mutants and focal adhesion. It is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. It may also play important roles in cell morphology and locomotion. Vinculin is expressed in the muscles. Mutations in the VCL gene may result in cardiomyopathy. STJ96247 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Vinculin protein.

Specificity: Vinculin Polyclonal Antibody detects endogenous levels of Vinculin protein.

Characteristics: Rabbit polyclonal to Vinculin.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Vinculin around the non-phosphorylation site of Y821.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000

Comment:

Metavinculin is muscle-specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin (VCL Products)

Synonyms: CMD1W antibody, CMH15 antibody, MV antibody, MVCL antibody, VCL antibody, im:6902790 antibody, hm:zehn2160 antibody, DDBDRAFT_0186762 antibody, DDBDRAFT_0232320 antibody, DDB_0186762 antibody, DDB_0232320 antibody, 9430097D22 antibody, AA571387 antibody, AI462105 antibody, AW545629 antibody, CG3299 antibody, CT11081 antibody, Dmel\\CG3299 antibody, Dmvincp antibody, EG:103B4.1 antibody, Vin antibody, Vin2EF antibody, vinc antibody, LOC100228062 antibody, vcl-A antibody, vinculin antibody, vinculin a antibody, vinculin A antibody, Vinculin antibody, vinculin S homeolog antibody, VCL antibody, vcla antibody, vinA antibody, Vcl antibody, Vinc antibody, CpipJ_CPIJ013873 antibody, Bm1_37935 antibody, vcl antibody, vcl.S antibody

Molecular Weight: 123 kDa

Gene ID: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2