IRF3 antibody (pSer385)

Catalog No. ABIN6287990

Product Details anti-IRF3 Antibody

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Binding Specificity: AA 320-400, pSer385

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: IRF-3 is a protein encoded by the IRF3 gene which is approximately 47,2 kDa. IRF-3 is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling and toll-like receptor signalling pathway. This protein falls under the interferon regulatory transcription factor family. It is found in an inactive form in the cytoplasm that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex is then translocated to the nucleus where it activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. IRF-3 is expressed constitutively in a variety of tissues. Mutations in the IRF3 gene may be involved in herpes simplex encephalitis. STJ91040 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous IRF-3 protein which only binds about S385 when S385 is phosphorylated.

Specificity: Phospho-IRF-3 (S385) Polyclonal Antibody detects endogenous levels of IRF-3 protein only when phosphorylated at S385.

Characteristics: Rabbit polyclonal to Phospho-IRF-3 (S385).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human IRF-3 around the phosphorylation site of S385.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000

Comment:

Expressed constitutively in a variety of tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: IRF-3 (IRF3 Products)

Synonyms: irf3 antibody, IRF3 antibody, C920001K05Rik antibody, IRF-3 antibody, interferon regulatory factor 3 antibody, interferon regulatory factor 7 antibody, irf3 antibody, IRF3 antibody, irf-3 antibody, Irf3 antibody, IRF7 antibody

Molecular Weight: 60 kDa

Gene ID: 3661

UniProt: Q14653

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades