Retinoblastoma 1 antibody (AA 703-772)

Catalog No. ABIN6294580

This Mouse Monoclonal antibody specifically detects Retinoblastoma 1 in WB, IHC and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for Retinoblastoma 1 antibody (AA 703-772) (ABIN6294580)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-Retinoblastoma 1 Antibody

Binding Specificity: AA 703-772

Purpose: Mouse anti-Human/Mouse Retinoblastoma / Rb Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: A human recombinant partial protein was used as the immunogen for this Rb antibody. The epitope is localized within amino acids 703-772.

Immunogen: A human recombinant partial protein was used as the immunogen for this Rb antibody. The epitope is localized within amino acids 703-772.

Application Details

Application Notes: WB: 0.5-1 μg/mL
IP: 1-2 μg/500 μg protein lysate
IHC (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Retinoblastoma-associated protein

Background:

Target Description: The retinoblastoma protein (protein name abbreviated pRb, gene name abbreviated RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, becomes inactive and allows cell cycle progression. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases.Rb restricts the cell's ability to replicate DNA by preventing its progression from the G1 (first gap phase) to S (synthesis phase) phase of the cell division cycle. Rb binds and inhibits transcription factors of the E2F family, which are composed of dimers of an E2F protein and a dimerization partner (DP) protein. The transcription activating complexes of E2 promoter-binding -protein-dimerization partners (E2F-DP) can push a cell into S phase. As long as E2F-DP is inactivated, the cell remains stalled in the G1 phase. When Rb is bound to E2F, the complex acts as a growth suppressor and prevents progression through the cell cycle. The Rb-E2F/DP complex also attracts a histone deacetylase (HDAC) protein to the chromatin, reducing transcription of S phase promoting factors, further suppressing DNA synthesis.[Wiki]mAb 1F8 ecognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein.

Gene Symbol: RB1

Gene ID: 5925

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy