BDNF antibody

Catalog No. ABIN636731

The Rabbit Polyclonal anti-BDNF antibody is suitable to detect BDNF in samples from Human. It has been validated for WB, ELISA and InhibAb.

Quick Overview for BDNF antibody (ABIN636731)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BDNF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Blocking Antibody (InhibAb)

Product Details anti-BDNF Antibody

Purification: Purified

Immunogen: BDNF antibody was raised in rabbit using highly pure recombinant human BDNF as the immunogen.

Application Details

Application Notes: ELISA: 0.5-2 µg/mL, Inhibition: 0.54-0.81 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background: BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/tr kb. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues.

Molecular Weight: 27 kDa (predicted detection band MW)

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity