HNF4A antibody (Internal Region)

Catalog No. ABIN6391399

Product Details anti-HNF4A Antibody

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Purpose: HNF4A

Sequence: HPHLMQEHMG TN

Specificity: This antibody is expected to recognise isoforms 1, 2, 4, 5, 7, 8 and 9

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments showed an approx 55 kDa band in Human Colon, Duodenum, Kidney and Liver lysates (calculated MW of 52.8 kDa according to NP_000448.3). Recommended concentration:0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4A (HNF4A Products)

Synonyms: HNF4A antibody, HNF4alpha antibody, CG9310 antibody, DmHNF4 antibody, Dmel\\CG9310 antibody, HNF-4 antibody, HNF-4(D) antibody, HNF4 antibody, Hnf-4 antibody, Hnf-4h antibody, NR2A4 antibody, dHNF-4 antibody, dHNF4 antibody, Hnf4 antibody, Hnf4alpha antibody, MODY1 antibody, Nr2a1 antibody, Tcf14 antibody, HNF4a7 antibody, HNF4a8 antibody, HNF4a9 antibody, MODY antibody, NR2A1 antibody, NR2A21 antibody, TCF antibody, TCF14 antibody, HNF-4alpha antibody, fb58h09 antibody, id:ibd1279 antibody, wu:fb58h09 antibody, hnf4 antibody, nr2a1 antibody, hepatocyte nuclear factor 4 alpha antibody, Hepatocyte nuclear factor 4 antibody, hepatic nuclear factor 4, alpha antibody, hepatocyte nuclear factor 4, alpha antibody, hepatocyte nuclear factor 4 alpha S homeolog antibody, HNF4A antibody, hnf4a antibody, Hnf4 antibody, Hnf4a antibody, hnf4a.S antibody

Background: HNF4A, hepatocyte nuclear factor 4, alpha , FLJ39654, HNF4, HNF4a7, HNF4a8, HNF4a9, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14, HNF4-alpha, hepatic nuclear factor 4 alpha, hepatocyte nuclear factor 4 alpha, transcription factor-14

Gene ID: 3172

NCBI Accession: NP_849180, NP_000448, NP_787110, NP_001025174, NP_001245284, NP_001274111, NP_001274112

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process