MGMT antibody (AA 8-221)

Catalog No. ABIN6560107

The Mouse Monoclonal anti-MGMT antibody has been validated for WB, FACS and IHC (p). It is suitable to detect MGMT in samples from Human.

Quick Overview for MGMT antibody (AA 8-221) (ABIN6560107)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MT3-1

Product Details anti-MGMT Antibody

Binding Specificity: AA 8-221

Specificity: Species Reactivity: Human. Cross-reacts weakly with mouse, rat and hamster. Other species not tested. Molecular Weight: .25 kDa Cellular Location: Nuclear and cytoplasmic.Positive Control: Raji cells, Colon carcinoma.

Immunogen: Purified recombinant human MGMT protein.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry Immunohistochemistry: (formalin / paraffin) Use at a 1:20 - 1:40 dilution for 60 minutes at room temperature (RT). Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10 - 20 minutes followed by cooling at RT for 20 minutes. Note: IHC requires the use of a high-efficiency detection system. Western blot: Use at 0.5-1 µg/mL for 2 hours at RT. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 500 µL of monoclonal antibody at 200 µg/mL in 10 mM PBS, pH 7.4, with protein stabilizer and 0.09% sodium azide. Purified from ascites fluid by Protein G chromatography.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background: Cancer chemotherapeutic alkylating agents (e.g. BCNU) act by inducing formation of lethal cross-links at the O6-alkylguanine position in DNA. MGMT (O6-methylguanine-DNA methyltransfer-ase) transfers alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug-resistant.

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus