MGMT antibody (O6-Methylguanine-DNA-Methyltransferase) (AA 8-221)

Details for Product anti-MGMT Antibody No. ABIN6560107
Antigen
Epitope
AA 8-221
23
18
18
5
5
4
4
4
3
3
2
1
1
1
1
1
1
1
Reactivity
Human
178
40
21
5
5
2
2
2
2
1
1
1
Host
Mouse
131
56
1
Clonality (Clone)
Monoclonal ()
Conjugate
This MGMT antibody is un-conjugated
8
6
5
3
3
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
158
67
64
35
30
29
22
6
4
3
2
1
1
1
Options
Immunogen Purified recombinant human MGMT protein.
Clone MT3-1
Isotype IgG1
Specificity Species Reactivity: Human. Cross-reacts weakly with mouse, rat and hamster. Other species not tested. Molecular Weight: .25 kDa Cellular Location: Nuclear and cytoplasmic.Positive Control: Raji cells, Colon carcinoma.
Antigen
Alternative Name MGMT (MGMT Antibody Abstract)
Background Cancer chemotherapeutic alkylating agents (e.g. BCNU) act by inducing formation of lethal cross-links at the O6-alkylguanine position in DNA. MGMT (O6-methylguanine-DNA methyltransfer-ase) transfers alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug-resistant.
Pathways DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
Application Notes Flow cytometry Immunohistochemistry: (formalin / paraffin) Use at a 1:20 - 1:40 dilution for 60 minutes at room temperature (RT). Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10 - 20 minutes followed by cooling at RT for 20 minutes. Note: IHC requires the use of a high-efficiency detection system. Western blot: Use at 0.5-1 µg/mL for 2 hours at RT. The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only
Format Liquid
Buffer 500 µL of monoclonal antibody at 200 µg/mL in 10 mM PBS, pH 7.4, with protein stabilizer and 0.09% sodium azide. Purified from ascites fluid by Protein G chromatography.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Image no. 1 for anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 8-221) antibody (ABIN6560107) anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 8-221) antibody (Image 1)
Image no. 2 for anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 8-221) antibody (ABIN6560107) anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 8-221) antibody (Image 2)