Recombinant BRAF antibody (V600E)

Catalog No. ABIN5647057

The Rabbit Monoclonal anti-BRAF antibody has been validated for WB, IHC and ICC. It is suitable to detect BRAF in samples from Human.

Quick Overview for Recombinant BRAF antibody (V600E) (ABIN5647057)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: RM8

Product Details anti-BRAF Antibody

Binding Specificity: V600E

Characteristics: This antibody reacts to the BRAF V600E mutant. No cross reactivity seen with wild type BRAF.

Purification: Purified

Purity: Protein A purified from animal origin-free supernatant

Immunogen: A peptide corresponding to BRAF V600E mutant was used as the immunogen for the recombinant BRAF V600E antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant BRAF V600E antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunohistochemistry: (FFPE): 0.5-5 μg/mL,Immunocytochemistry: 2-10 μg/mL,Western Blot: 0.5-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in PBS with 50 % Glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant BRAF V600E antibody at -20°C.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF V600E

Background: This antibody reacts to the BRAF V600E mutant. No cross reactivity seen with wild type BRAF.

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy