MCM3 antibody (Minichromosome Maintenance Complex Component 3)

Details for Product anti-MCM3 Antibody No. ABIN6567003
Antigen
Reactivity
Human, Monkey, Mouse (Murine)
136
66
60
8
6
4
4
4
3
2
1
1
1
1
Host
Rabbit
120
15
2
Clonality
Polyclonal
Conjugate
This MCM3 antibody is un-conjugated
7
5
5
5
5
5
5
3
3
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
103
52
36
26
19
16
8
7
5
4
3
2
2
1
1
Options
Immunogen Recombinant protein of human MCM3
Isotype IgG
Purification Affinity purification
Antigen
Alternative Name MCM3 (MCM3 Antibody Abstract)
Background

Synonyms: Cervical cancer proto oncogene 5,DNA polymerase alpha holoenzyme associated P1,DNA polymerase alpha holoenzyme associated protein P1,DNA polymerase alpha holoenzyme-associated protein P1,DNA replication factor MCM3,DNA replication licensing factor mcm3,HCC 5,HCC5,hRlf beta subunit,Human cervical cancer proto oncogene 5,MCM 3,mcm3,MCM3 minichromosome maintenance deficient 3,MCM3,MGC1157,Minichromosome maintenance complex component 3,Minichromosome maintenance deficient 3,Minichromosome maintenance protein 3,P1 h,P1 MCM3,P1 Protein,P1-MCM3,P1.h,p102,P102 protein,Replication licensing factor beta subunit,RLF beta subunit,RLF subunit beta,RLFB

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight

Observed_MW: 115kDa

Calculated_MW: 90kDa/95kDa

Gene ID 4172
UniProt P25205
Pathways DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze / thaw cycles.
Supplier Images
Image no. 1 for anti-Minichromosome Maintenance Complex Component 3 (MCM3) antibody (ABIN6567003) Immunohistochemistry of paraffin-embedded human oophoroma using MCM3 antibody.
Image no. 2 for anti-Minichromosome Maintenance Complex Component 3 (MCM3) antibody (ABIN6567003) Immunofluorescence analysis of U2OS cells using MCM3 antibody.
Image no. 3 for anti-Minichromosome Maintenance Complex Component 3 (MCM3) antibody (ABIN6567003) Western blot analysis of extracts of various cell lines, using MCM3 antibody.