Amyloid Fibrils antibody

Catalog No. ABIN6655141

Product Details

Target: Amyloid Fibrils

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purification: Anti-Amyloid Fibrils (OC) Antibody was purified by Protein A chromatography. A BLAST analysis was used to suggest cross-reactivity with Amyloid Fibrils (OC) from Human based on 100% homology with the immunizing sequence. Expected to detect in mouse and rat based on species homology. Recognizes generic epitopes common to many amyloid fibrils and fibrillar oligomers, but not prefibrilllar oligomers or natively folded proteins. Cross-reactivity with Amyloid Fibrils (OC) from other sources has not been determined. Neuroscience research.

Immunogen:

Immunogen: Amyloid Fibrils (OC) Antibody was produced from whole rabbit serum prepared by repeated immunizations with fibrils prepared from human Abeta42 synthetic peptide.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Amyloid Fibrils (OC) Antibody is suitable for use in IP, IF microscopy, IHC, ELISA, WB, and Dot blot. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:200

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details

Target: Amyloid Fibrils

Alternative Name: Amyloid Fibrils (OC)

Background:

Synonyms: Amyloid OC, Fibrils, Amyloid Oligomer αβ, A11, Amyloid beta A4 protein, ABPP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, APP, A4, AD1

Background: Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres. These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease.

Gene Name: APP