EPAS1 antibody (Internal Region)

Catalog No. ABIN6655142

Product Details anti-EPAS1 Antibody

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purification: Anti-HIF2α Antibody was purified by Protein G chromatography. This monoclonal antibody is specific for human HIF2α protein. A BLAST analysis was used to suggest cross-reactivity with HIF2α from human based on 100% homology with the immunizing sequence. Cross-reactivity with HIF2α from other sources has not been determined. Oxidative Stress/Cell Signaling research.

Immunogen:

Immunogen: HIF2 alpha Antibody was produced in mice by repeated immunizations raised against a recombinant protein corresponding to an internal region of human EPAS-1 (HIF2alpha).

Immunogen Type: Recombinant Protein

Clone: EP190B

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-HIF2α Antibody is recommended for use in ELISA, WB, IHC and IF microscopy. Antibody is provided in PBS pH 7.4. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:200

Western Blot Dilution: 1:100 - 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer:

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: HIF2 alpha (EPAS1 Products)

Synonyms: EPAS1 antibody, ECYT4 antibody, HIF2A antibody, HLF antibody, MOP2 antibody, PASD2 antibody, bHLHe73 antibody, HIF-2alpha antibody, HRF antibody, Hif2a antibody, bhlhe73 antibody, ecyt4 antibody, hif2 antibody, hif2a antibody, hif2alpha antibody, hlf antibody, mop2 antibody, pasd2 antibody, EPAS antibody, EPAS-1 antibody, endothelial PAS domain protein 1 antibody, endothelial PAS domain protein 1 L homeolog antibody, EPAS1 antibody, epas1 antibody, Epas1 antibody, epas1.L antibody

Background:

Synonyms: mouse anti-HIF2 alpha Antibody, mouse anti-HIF2a Antibody, ECYT4, EPAS1, HIF2alpha, HIF1 Alpha like factor, HLF, Hypoxia inducible factor 2 alpha, MOP2, PASD2, Endothelial PAS domain-containing protein 1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73

Background: Members of the hypoxia-inducible factor (HIF) family of transcription factors regulate the cellular response to hypoxia. HIF2-Alpha is involved in catecholamine homeostasis, vascular remodeling, physiological angiogenesis and adipogenesis. It is overexpressed in many cancerous tissues, but its exact role in tumour progression remains to be clarified. Studies suggest that in the case of non-small cell lung cancer, HIF2-Alpha actually is a promoter of tumor growth and progression in a solid tumor. Other data suggests that HIF2-Alpha is an important regulator of innate immunity, and therefore may be useful in the therapeutic target for treating inflammatory disorders and cancer.

Gene Name: EPAS1

Gene ID: 2034

NCBI Accession: NP_001421

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect