LC3B antibody (N-Term)
-
- Target See all LC3B (MAP1LC3B) Antibodies
- LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
-
Binding Specificity
- N-Term
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This LC3B antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Cross-Reactivity
- Human, Mouse (Murine), Rat (Rattus)
- Purification
- Anti-ATG8 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-ATG8 from Opossum and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-ATG8 from other sources has not been determined.
- Immunogen
-
Immunogen: ATG8 Antibody was produced from whole rabbit serum prepared by repeated immunizations with at the n-terminus portion of human ATG8.
Immunogen Type: Peptide
- Isotype
- IgG
- Top Product
- Discover our top product MAP1LC3B Primary Antibody
-
-
- Application Notes
-
Immunohistochemistry Dilution: 5-10 μg/mL
Application Note: Anti-ATG8 antibody is suitable for use in IHC (p) and WB. Expect a band approximately 16 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 0.25-1 μg/mL
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.05 % BSA
0.05 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
- Target
- LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
- Alternative Name
- ATG8 (MAP1LC3B Products)
- Synonyms
- ATG8F antibody, LC3B antibody, MAP1A/1BLC3 antibody, MAP1LC3B-a antibody, Map1lc3 antibody, Mpl3 antibody, zbs559 antibody, 1010001C15Rik antibody, Atg8 antibody, LC3b antibody, zgc:56434 antibody, wu:fb60g11 antibody, map1lc3b antibody, MGC76283 antibody, MAP1LC3B antibody, microtubule associated protein 1 light chain 3 beta antibody, microtubule-associated protein 1 light chain 3 beta antibody, microtubule associated protein 1 light chain 3 beta L homeolog antibody, MAP1LC3B antibody, Map1lc3b antibody, map1lc3b antibody, map1lc3b.L antibody
- Background
-
Synonyms: Activating transcription factor 8
Background: Anti-ATG8 antibody detects human ATG8. MAP1A and MAP1B are microtubule-associated proteins made up of a heavy chain and multiple light chain components. One such light chain component associated with these proteins is ATG8. ATG8 (also called MAP1LC3A and APG8), an ~16KDa mammalian homolog of yeast Atg8, is a cytoplasmic protein that plays an essential role in the process and regulation of autophagy. ATG8 is an evolutionarily conserved protein that belongs to LC3 family. ATG8 interacts with various proteins in the cytoplasm including mammalian homologues of Atg4, Atg7, Atg3 and Atg12 and gets modified into LC3-I and LC3-II respectively. Both of these forms are suggested to be involved in the formation and intracellular trafficking of autophagosomes in mammalian cells. Anti-ATG8 antibody are ideal for investigators invovled in apoptosis research.
Gene Name: MAP1LC3A
- Gene ID
- 84557
- NCBI Accession
- NP_115903
- UniProt
- Q9H492
- Pathways
- Autophagy
-