Nav1.8 antibody (C-Term)

Catalog No. ABIN6658124

Product Details anti-Nav1.8 Antibody

Target: Nav1.8 (SCN10A) (Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A))

Binding Specificity: C-Term

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nav1.8 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-NAV1.8 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with NAV1.8 from human, mouse, and rat based on 100% homology with the immunizing sequence. No cross reactivity against other Nav channels. Cross-reactivity with NAV1.8 from other sources has not been determined. Ion Channels research.

Immunogen:

Immunogen: Nav1.8 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding near the cytoplasmic C-terminus region of rat Nav1.8.

Immunogen Type: Recombinant Protein

Clone: S134-12

Isotype: IgG2a

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-NAV1.8 Antibody is suitable for use in WB, IHC and IF microscopy. Expect a band approximately ~220 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1-10 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for Nav1.8

Target: Nav1.8 (SCN10A) (Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A))

Alternative Name: NAV1.8 (SCN10A Products)

Synonyms: Nav1.8 antibody, PN3 antibody, SNS antibody, hPN3 antibody, Na(V)1.8 antibody, sodium channel, voltage-gated, type X, alpha antibody, sodium voltage-gated channel alpha subunit 10 antibody, Scn10a antibody, SCN10A antibody

Background:

Synonyms: PN3, Na(V)1.8, Scn10a, Sodium channel protein type 10 subunit alpha, Peripheral nerve sodium channel 3, Sensory neuron sodium channel, Sodium channel protein type X subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.8

Background: Nav1.8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.8 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.8 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals.

Gene Name: Scn10a

Gene ID: 29571

NCBI Accession: NP_058943

UniProt: Q62968