Synaptotagmin antibody (pSer309)

Catalog No. ABIN6658255

Product Details anti-Synaptotagmin Antibody

Target: Synaptotagmin (SYT)

Binding Specificity: pSer309

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptotagmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purification: Anti-Synaptotagmin 1 pS309 Antibody is directed against rat Synaptotagmin 1 phosphorylated at pS309. Synaptotagmin antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity is expected from the following species based on 100% sequence homology: bovine, canine, chicken, human, mouse, non-human primate and zebra fish. Cross-reactivity with from other species has not been determined.

Immunogen:

Immunogen: Anti-Synaptotagmin pS309 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 of Synaptotagmin.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:400

Application Note: Anti-Synaptotagmin pS309 antibody is suitable for use in Western Blotting and IHC. Expect a band of approximately 60-62 kDa in size corresponding to synaptotagmin protein phosphorylated at Ser309 in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details for Synaptotagmin

Target: Synaptotagmin (SYT)

Alternative Name: Synaptotagmin (SYT Products)

Synonyms: Syti antibody, GB20036 antibody, DKFZP459P193 antibody, SYT1 antibody, SytI antibody, SyNapTotagmin antibody, synaptotagmin antibody, synaptotagmin 1 antibody, snt-5 antibody, snt-6 antibody, snt-3 antibody, Syt antibody, Syt1 antibody, SYT1 antibody

Background:

Synonyms: SytI, p65

Background: Synaptotagmin 1 pS309 Antibody detects Synaptotagmin 1, which is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS. It is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Recent studies indicate that synaptotagmin's Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion. It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin's ability to influence both the exocytotic and endocytotic components of synaptic transmission. Anti-Synaptotagmin 1 pS309 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: SYT1

Gene ID: 25716

UniProt: P21707