PDK4 antibody (AA 311-411)

Catalog No. ABIN671856

This anti-PDK4 antibody is a Rabbit Polyclonal antibody detecting PDK4 in WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). Suitable for Human and Mouse.

Quick Overview for PDK4 antibody (AA 311-411) (ABIN671856)

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PDK4 Antibody

Binding Specificity: AA 311-411

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Pdk4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PDK4

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Alternative Name: PDK4

Background:

Synonyms: FLJ40832, Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial, Pyruvate dehydrogenase kinase 4, Pyruvate dehydrogenase kinase isoform 4, Pyruvate dehydrogenase kinase isozyme 4 mitochondrial.

Background: Pdk4 (pyruvate dehydrogenase kinase, isozyme 4) mediates glucose oxidation through inhibitory phosphorylation of the pyruvate dehydrogenase complex. The pyruvate dehydrogenase complex (PDC) is a mitochondrial matrix enzyme that plays an key role in aerobic energy metabolism by catalyzing the oxidative decarboxylation of pyruvate to form acetyl-CoA for subsequent breakdown in the Krebs cycle. The enzymatic activity of the mammalian PDC is regulated through phosphorylation and desphosphorylation of the E1?subunit of the complex. The enzymes responsible for the phosphorylation and inactivation of the PDC are pyruvate dehydrogenase kinases (PDK1-4). [TISSUE SPECI FICITY] Ubiquitous, highest levels of expression in heart and skeletal muscle. Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain.

Gene ID: 5166

Pathways: PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect