CD14 antibody (AA 201-300)

Catalog No. ABIN673164

Product Details anti-CD14 Antibody

Target: CD14

Binding Specificity: AA 201-300

Reactivity: Human, Mouse, Rat, Dog, Sheep, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Dog, Human, Mouse, Rabbit, Rat, Sheep

Predicted Reactivity: Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse CD14

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD14 antibody (ABIN673164)

Kulhankova, Kinney, Stach, Gourronc, Grumbach, Klingelhutz, Salgado-Pabón: "The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function." in: Infection and immunity, (2018) (PubMed).

Lee, Chen, Wallace, Sung, Sheu, Chung, Chen, Lu, Ko, Sun, Chiang, Chang, Lee, Yip: "Xenogeneic human umbilical cord-derived mesenchymal stem cells reduce mortality in rats with acute respiratory distress syndrome complicated by sepsis." in: Oncotarget, Vol. 8, Issue 28, pp. 45626-45642, (2018) (PubMed).

Sun, Chen, Chang, Chiang, Sung, Chen, Chen, Chen, Kao, Chang, Lee, Yip: "Melatonin treatment enhances therapeutic effects of exosomes against acute liver ischemia-reperfusion injury." in: American journal of translational research, Vol. 9, Issue 4, pp. 1543-1560, (2017) (PubMed).

Chang, Hsu, Yang, Sung, Chen, Huang, Kao, Chen, Chen, Chiang, Yip, Lee: "Enhanced protection against renal ischemia-reperfusion injury with combined melatonin and exendin-4 in a rodent model." in: Experimental biology and medicine (Maywood, N.J.), Vol. 241, Issue 14, pp. 1588-602, (2017) (PubMed).

Nau, Seebach, Trumm, Schaible, Kontradowitz, Meier, Buechner, Marzi, Henrich: "Alteration of Masquelet's induced membrane characteristics by different kinds of antibiotic enriched bone cement in a critical size defect model in the rat's femur." in: Injury, Vol. 47, Issue 2, pp. 325-34, (2016) (PubMed).

Chen, Yu, Fan, Bian, Ma, Lu, Jin: "Sargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-?B) and induces cytokine production via Toll-like receptors." in: Carbohydrate polymers, Vol. 105, pp. 113-20, (2014) (PubMed).

Chen, Chiang, Chen, Sung, Lee, Tsai, Chang, Chen, Sun, Leu, Chang, Yang, Yip: "Melatonin treatment further improves adipose-derived mesenchymal stem cell therapy for acute interstitial cystitis in rat." in: Journal of pineal research, Vol. 57, Issue 3, pp. 248-61, (2014) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Synonyms: CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background:

Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14

Background: In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity).

Gene ID: 12475

UniProt: P10810

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades