FOXO3 antibody (C-Term)

Catalog No. ABIN6736383

The Rabbit Polyclonal anti-FOXO3 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect FOXO3 in samples from Human, Mouse, Rat, Dog, Horse, Chicken, Guinea Pig, Monkey, Bat and Hamster.

Quick Overview for FOXO3 antibody (C-Term) (ABIN6736383)

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Reactivity: Human, Mouse, Rat, Dog, Horse, Chicken, Guinea Pig, Monkey, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXO3 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human FOXO3 / FOXO3A

Predicted Reactivity: Percent identity by BLAST analysis: Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human FOXO3 (Q68DV3). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Lizard (100%), Galago, Sheep, Bovine, Rabbit, Pig, Platypus (92%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FOXO3 / FOXO3A

Background: Name/Gene ID: FOXO3
Family: Transcription factor

Synonyms: FOXO3, AF6q21, AF6q21 protein, Forkhead box O3, Forkhead box O3A, FOXO3A, FKHRL1P2, Forkhead box protein O3, FOXO2, FKHRL1

Gene ID: 2309

UniProt: O43524

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis