RASGRF1 antibody (AA 107-156)

Catalog No. ABIN6744091

The Rabbit Polyclonal anti-RASGRF1 antibody has been validated for WB. It is suitable to detect RASGRF1 in samples from Mouse, Human, Cow, Dog, Guinea Pig, Monkey and Pig.

Quick Overview for RASGRF1 antibody (AA 107-156) (ABIN6744091)

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Reactivity: Mouse, Human, Cow, Dog, Guinea Pig, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASGRF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RASGRF1 Antibody

Binding Specificity: AA 107-156

Specificity: Human RASGRF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Pig (100%) Rabbit, Horse, Chicken, Zebrafish (92%) Mouse (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa107-156 of human RASGRF1 (Q13972-2, NP_722522). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Elephant, Dog, Bovine, Pig, Opossum, Guinea pig (100%), Rabbit, Horse, Turkey, Chicken, Xenopus, Zebrafish (92%), Mouse (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RASGRF1

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Alternative Name: RASGRF1 / CDC25

Background: Name/Gene ID: RASGRF1

Synonyms: RASGRF1, CDC25, CDC25L, GNRP, GRF55, H-GRF55, PP13187, CDC25 homolog, GRF1, Ras-GRF1

Gene ID: 5923

NCBI Accession: NP_722522

UniProt: Q13972

Pathways: RTK Signaling, Cell Division Cycle, M Phase