PDK2 antibody (AA 251-300)

Catalog No. ABIN6746981

This Rabbit Polyclonal antibody specifically detects PDK2 in WB, IHC and IHC (p). It exhibits reactivity toward Human and Cow.

Quick Overview for PDK2 antibody (AA 251-300) (ABIN6746981)

Target: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDK2 Antibody

Binding Specificity: AA 251-300

Brand: IHC-plus™

Specificity: Human PDK2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Rabbit, Horse, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of human PDK2 (Q15119, NP_002602). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon (100%), Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Rabbit, Horse, Opossum, Guinea pig (92%), Bat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PDK2

Target: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Alternative Name: PDK2

Background: Name/Gene ID: PDK2
Subfamily: PDK/BCKDK
Family: Protein Kinase

Synonyms: PDK2, PDHK2, PDHKII

Gene ID: 5164

NCBI Accession: NP_002602

UniProt: Q15119

Pathways: PI3K-Akt Signaling, RTK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect