GZMB antibody (AA 21-130)

Catalog No. ABIN675354

Cited in 1 publication. The Rabbit Polyclonal anti-GZMB antibody (ABIN675354) specifically detects GZMB in WB, ELISA, FACS, IHC (p), IF (p), IHC (fro) and IF (cc). The antibody is reactive with Human and Mouse samples.

Quick Overview for GZMB antibody (AA 21-130) (ABIN675354)

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-GZMB Antibody

Binding Specificity: AA 21-130

Specificity: Specific towards Granzyme B and Granzyme H

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GZMB

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-GZMB antibody (ABIN675354)

Oláh, Kálmán, Tóth, Zvara, Sántha, Ivitz, Janka, Pákáski: "Proteomic Analysis of Cerebrospinal Fluid in Alzheimer's Disease: Wanted Dead or Alive." in: Journal of Alzheimer's disease : JAD, (2014) (PubMed).

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: Granzyme B

Background:

Synonyms: HLP, CCPI, CGL1, CSPB, SECT, CGL-1, CSP-B, CTLA1, CTSGL1, Granzyme B, C11, CTLA-1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, T-cell serine protease 1-3E, GZMB, GRB, Granzyme B, Granzyme H

Background: This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Gene ID: 3002

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis