mGluR2/3 antibody (AA 864-883)

Catalog No. ABIN6757035

Product Details anti-mGluR2/3 Antibody

Target: mGluR2/3 (GRIA2/3) (Glutamate Receptor, Ionotropic, AMPA 2/3 (GRIA2/3))

Binding Specificity: AA 864-883

Reactivity: Human, Rat, Mouse, Chicken, Zebrafish (Danio rerio), Horse, Monkey, Bat, Rabbit, Cow, Dog, Xenopus laevis, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This mGluR2/3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Recognizes rat GluR2/3, Mr 110kD. Species cross-reactivity: human, mouse, monkey and chicken.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Zebra finch, Chicken, Platypus, Xenopus, Pufferfish, Zebrafish (100%) Stickleback (90%) Turkey (85%).

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to aa864-883 (C-QNFATYKEGYNVYGIESVKI) of rat GluR2 (KLH coupled). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Zebra finch, Chicken, Platypus, Xenopus, Pufferfish, Zebrafish (100%), Stickleback (90%), Turkey (85%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation. Rat microsomal protein preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-GluR2/ 3 (1 μg/mL). Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg. Immunoprecipitates GluR2/3 from 1000 μg of rat brain membranes.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.1 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for mGluR2/3

Target: mGluR2/3 (GRIA2/3) (Glutamate Receptor, Ionotropic, AMPA 2/3 (GRIA2/3))

Alternative Name: GLUR2 + GLUR3 (GRIA2/3 Products)

Synonyms: glutamate ionotropic receptor AMPA type subunit 2 antibody, GRIA2 antibody

Background: This antibody detects: GLUR2 + GLUR3