ACTN2 antibody (AA 557-692)
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- Target See all ACTN2 Antibodies
- ACTN2 (Actinin, alpha 2 (ACTN2))
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Binding Specificity
- AA 557-692
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ACTN2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- A recombinant fragment (aa557-692) of human ACTN2 protein (exact sequence is proprietary)
- Clone
- ACTN2-3293
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product ACTN2 Primary Antibody
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- Application Notes
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Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Skeletal or Cardiac Muscle.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- ACTN2 (Actinin, alpha 2 (ACTN2))
- Alternative Name
- ACTN2 (ACTN2 Products)
- Synonyms
- MGC89234 antibody, ACTN2 antibody, 1110008F24Rik antibody, CMD1AA antibody, wu:fd44c03 antibody, zgc:123223 antibody, actinin alpha 2 antibody, actinin, alpha 2b antibody, actinin alpha 2 S homeolog antibody, actn2 antibody, ACTN2 antibody, Actn2 antibody, actn2b antibody, actn2.S antibody
- Background
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Actin binding protein, Alpha actinin skeletal muscle, isoform 2, CMD1AA, F-actin cross-linking protein,Sarcomeric Actinin Alpha 2 / ACTN2
Cellular localisation: Cytoplasmic - Molecular Weight
- 103kDa
- Gene ID
- 88, 498178
- UniProt
- P35609
- Pathways
- Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Negative Regulation of Transporter Activity
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