Iba1 antibody (AA 1-146)
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- Target See all Iba1 (IBA1) Antibodies
- Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
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Binding Specificity
- AA 1-146
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Iba1 antibody is un-conjugated
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Application
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- AIF1 is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Purified fragment of human recombinant AIF1 protein (around aa 1-146) (exact sequence is proprietary)
- Clone
- AIF1-1909
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product IBA1 Primary Antibody
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- Application Notes
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Lymph Node, Tonsil or Kidney.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
- Alternative Name
- AIF1 (IBA1 Products)
- Synonyms
- AIF-1 antibody, IBA1 antibody, IRT-1 antibody, IRT1 antibody, AI607846 antibody, D17H6S50E antibody, G1 antibody, Iba1 antibody, Bart1 antibody, iba1 antibody, mrf-1 antibody, fa04b11 antibody, wu:fa04b11 antibody, AIF antibody, AIF1 antibody, aif1 antibody, allograft inflammatory factor 1 antibody, AIF1 antibody, Aif1 antibody, aif1 antibody
- Background
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Allograft inflammatory factor 1 (AIF1), balloon angioplasty responsive transcription (BART1), IBA1, Interferon responsive transcript 1, Ionized calcium-binding adapter molecule 1, IRT1, Microglia response factor (MRF1),AIF1 / Iba1 (Microglia Marker)
Cellular localisation: Cytoplasmic and Cell Surface - Molecular Weight
- 17kDa
- Gene ID
- 199, 76364
- UniProt
- P55008
- Pathways
- Smooth Muscle Cell Migration
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