AKT1 antibody (AA 1-200)

Catalog No. ABIN6936378

The Mouse Monoclonal anti-AKT1 antibody is suitable to detect AKT1 in samples from Human. It has been validated for WB and IHC.

Quick Overview for AKT1 antibody (AA 1-200) (ABIN6936378)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: AKT1-2784

Product Details anti-AKT1 Antibody

Binding Specificity: AA 1-200

Purpose: AKT1 (Prognostic Marker for Neuroendocrine Tumors) Antibody

Specificity: Recognizes a protein of 62 kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB æ or RacPK- æ) and Akt 3 (also designated PKBÁ or thymoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR- æ tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.

Cross-Reactivity (Details): Human. Mouse.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human AKT1 protein (around aa 1-200) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: AKT1

Background:

AKT1, Oncogene AKT1, PKB, PKB-ALPHA, PRKBA, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC Alpha, RAC Serine/Threonine Protein Kinase, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma.

Molecular Weight: 62kDa

Gene ID: 207, 525622

UniProt: P31749

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect