AMACR antibody (AA 297-394)
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- Target See all AMACR Antibodies
- AMACR (alpha-Methylacyl-CoA Racemase (AMACR))
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Binding Specificity
- AA 297-394
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This AMACR antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- This antibody recognizes a protein of 42 kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant human AMACR protein fragment (around aa 297-394) (exact sequence is proprietary)
- Clone
- AMACR-1864
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product AMACR Primary Antibody
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- Application Notes
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Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: PC3 or HEK cells. Prostate Adenocarcinoma and human kidney tissue.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- AMACR (alpha-Methylacyl-CoA Racemase (AMACR))
- Alternative Name
- AMACR (AMACR Products)
- Synonyms
- AMACR antibody, DKFZp469O1232 antibody, amacr antibody, NCU04099.1 antibody, MGC89832 antibody, AMACRD antibody, CBAS4 antibody, RACE antibody, RM antibody, Macr1 antibody, Da1-8 antibody, Marc1 antibody, alpha-methylacyl-CoA racemase antibody, PROBABLE ALPHA-METHYLACYL-COA RACEMASE MCR (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase) antibody, Alpha-methylacyl-CoA racemase antibody, alpha-methylacyl-CoA racemase L homeolog antibody, AMACR antibody, mcr antibody, BPSL0064 antibody, Maqu_2037 antibody, UREG_03852 antibody, MCYG_01809 antibody, amacr antibody, amacr.L antibody, MGYG_04259 antibody, NCU04099 antibody, Amacr antibody
- Background
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Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM,AMACR / p504S (Prostate Cancer Marker)
Cellular localisation: Cytoplasmic (granular) - Molecular Weight
- 42kDa
- Gene ID
- 23600, 508343
- UniProt
- Q9UHK6
- Pathways
- Monocarboxylic Acid Catabolic Process
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