Cyclin A1 antibody

Catalog No. ABIN6936580

Product Details anti-Cyclin A1 Antibody

Target: Cyclin A1 (CCNA1)

Reactivity: Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin A1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F, and retinoblastoma protein p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. The antibody detects Xenopus cyclin A in blots. Cyclin A1 is the embryonic cyclin which is present in Xenopus eggs. This MAb only detects Xenopus Cyclin A1.

Cross-Reactivity (Details): Xenopus laevis

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length Xenopus laevis Cyclin A1 protein

Clone: XLA1-3

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Very high levels in testis. Also found in myeloid leukemia cell lines. Xenopus laevis tissue lysate.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Cyclin A1

Target: Cyclin A1 (CCNA1)

Alternative Name: ccna1 (CCNA1 Products)

Synonyms: fd14f08 antibody, wu:fd14f08 antibody, wu:fi20d12 antibody, zgc:111869 antibody, MGC81965 antibody, cyclin-A1 antibody, CCNA1 antibody, MGC132235 antibody, cyclin-A1-2 antibody, Cyclin-A1 antibody, ccna1 antibody, cyclin A1 antibody, cyclin A1 L homeolog antibody, CYCLIN A1;2 antibody, cyclin A1 S homeolog antibody, CCNA1 antibody, ccna1 antibody, ccna1.L antibody, CYCA1;2 antibody, Ccna1 antibody, ccna1.S antibody

Background: CCN1, CCNA, CCNA2, Cyclin A1,Cyclin A1
Cellular localisation: Nucleus.

Molecular Weight: 65kDa

Gene ID: 4267

UniProt: P18606

Pathways: Apoptosis, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA