COL7A1 antibody
-
- Target See all COL7A1 Antibodies
- COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This COL7A1 antibody is un-conjugated
-
Application
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
- Cross-Reactivity (Details)
- Human. Sheep. Goat. Horse. Guinea Pig. Cow.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human Collagen VII protein
- Clone
- LH7-2
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product COL7A1 Primary Antibody
-
-
- Application Notes
-
Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.
- Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
-
- Target
- COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))
- Alternative Name
- COL7A1 (COL7A1 Products)
- Synonyms
- COL7A1 antibody, EBD1 antibody, EBDCT antibody, EBR1 antibody, AW209154 antibody, collagen type VII alpha 1 chain antibody, collagen, type VII, alpha 1 antibody, COL7A1 antibody, col7a1 antibody, Col7a1 antibody
- Background
-
Alpha 1 type VII collagen, Collagen 7, Collagen alpha 1(VII) chain, Collagen type VII alpha 1, Collagen VII alpha 1 polypeptide Col7a1, EBD1, EBDCT, EBR1, Long-chain (LC) collagen,Collagen VII
Cellular localisation: Secreted. Extracellular matrix. Basement Membrane. - Molecular Weight
- 290kDa
- Gene ID
- 1294, 476218
- UniProt
- Q02388
-