RCAS1 antibody

Catalog No. ABIN6937120

Product Details anti-RCAS1 Antibody

Target: RCAS1 (EBAG9) (Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RCAS1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: EBAG9, also known as RCAS1, is an estrogen-transcribed protein. Soluble and membranous RCAS1 proteins may play a role in the immune escape of tumor cells by promoting T lymphocyte inhibition of growth and apoptosis. RCAS1 is expressed in a wide variety of cancers, including uterine, ovarian, and lung cancer cells, and acts as a ligand for a putative receptor present on peripheral lymphocytes. RCAS1 is highly expressed not only in cancer cells but also in non-tumor bile duct cells subject to immune attack. RCAS1 inhibits the in vitro growth of receptor-expressing cells and induces apoptosis, contributing to the ability of tumor cells to evade host immune surveillance. High expression of RCAS1 significantly correlates with tumor progression and with poor outcome for many cancer patients.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length EBAG9 protein

Clone: CPTC-EBAG9-1

Isotype: IgG2c kappa

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, Jurkat, 293 cell lysates, Human skeletal muscle tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for RCAS1

Target: RCAS1 (EBAG9) (Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))

Alternative Name: EBAG9 (EBAG9 Products)

Synonyms: EB9 antibody, PDAF antibody, MGC56608 antibody, zgc:56608 antibody, MGC84425 antibody, EBAG9 antibody, AI835379 antibody, Rcas1 antibody, RCAS1 antibody, eb9 antibody, pdaf antibody, rcas1 antibody, estrogen receptor binding site associated, antigen, 9 antibody, estrogen receptor binding site associated, antigen, 9 L homeolog antibody, estrogen receptor-binding fragment-associated gene 9 antibody, EBAG9 antibody, ebag9 antibody, ebag9.L antibody, Ebag9 antibody

Background: BAG9, cancer associated surface antigen, cancer associated surface antigen RCAS1, EB9, estrogen receptor binding fragment associated gene 9, PDAF, receptor binding cancer antigen expressed on SiSo cells,RCAS1 / Estrogen Receptor Binding Site Associated, Antigen 9
Cellular localisation: Golgi apparatus.

Molecular Weight: 32kDa

Gene ID: 9166, 409368

UniProt: O00559