HLA-DR antibody

Catalog No. ABIN6937409

This Mouse Monoclonal antibody specifically detects HLA-DR in FACS, WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for HLA-DR antibody (ABIN6937409)

Target: HLA-DR

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DR antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: SPM289

Product Details anti-HLA-DR Antibody

Purpose: HLA-DR (MHC II) Antibody

Specificity: This MAb reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Cross-Reactivity (Details): Human. Others not known.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Activated human peripheral blood mononuclear cells

Isotype: IgG3, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistology (Frozen & Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HLA-DR

Target: HLA-DR

Alternative Name: HLA-DR

Background:

DRB1, DRB4, HLA class II histocompatibility antigen DR alpha chain, HLA DR1B, HLA DR3B, HLA DRA, HLA DRA1, HLA DRB1, HLA DRB3, HLA DRB4, HLA DRB5, HLA-DRA, HLADR4B, HLADRA1, HLADRB, Major histocompatibility complex class II DR beta 1 / 3/ 4, Major histocompatibility complex class II DR beta 5, MHC cell surface glycoprotein

Daudi or HuT78 cells. Human tonsil or lymph node., Ramos

Molecular Weight: ~28kDa (beta chain)

Gene ID: 3122, 520048

UniProt: P01903

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response