ICAM-3/CD50 antibody
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- Target See all ICAM-3/CD50 (ICAM3) Antibodies
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ICAM-3/CD50 antibody is un-conjugated
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Application
- Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Binding Studies (Bind)
- Specificity
- Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Stimulated human leukocytes
- Clone
- 186-2G9
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product ICAM3 Primary Antibody
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anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody
ICAM3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-171 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibodyICAM3 Reactivity: Human FACS, IHC (p), Func Host: Mouse Monoclonal MEM-04 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-203) antibodyICAM3 Reactivity: Human WB, FACS, ELISA, IHC, ICC, Neut Host: Mouse Monoclonal 2H10G7 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 200-547) antibodyICAM3 Reactivity: Human WB, IF, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody (Biotin)ICAM3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-171 Biotin
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody (PE)ICAM3 Reactivity: Human FACS Host: Mouse Monoclonal MEM-171 PE
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-547) antibodyICAM3 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 2F8 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 46-197) antibodyICAM3 Reactivity: Human WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 259-491) antibodyICAM3 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-130) antibodyICAM3 Reactivity: Human ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Known_Application: Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (Order Ab without Azide),Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HL-60 or THP-1 cells. Lymph node and tonsil.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- Alternative Name
- ICAM3 (ICAM3 Products)
- Synonyms
- CD50 antibody, CDW50 antibody, ICAM-R antibody, ICAM-3 antibody, ICAM3 antibody, intercellular adhesion molecule 3 antibody, ICAM3 antibody, Icam3 antibody
- Background
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ICAMR, Intercellular adhesion molecule 3 (ICAM3),CD50 / ICAM3
Cellular localisation: Cell surface - Molecular Weight
- 110-160kDa
- Gene ID
- 3385, 354563
- UniProt
- P32942
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