IGHM antibody

Catalog No. ABIN6937515

Product Details anti-IGHM Antibody

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGHM antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant heavy chain of human IgM

Clone: IM260

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Raji or hPBL cells. Human tonsil or spleen (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for IGHM

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Alternative Name: IGHM (IGHM Products)

Synonyms: AI326478 antibody, Igh-6 antibody, Igh-M antibody, Igh6 antibody, Igm antibody, muH antibody, ighm1 antibody, wu:fj14a08 antibody, zgc:101769 antibody, AGM1 antibody, MU antibody, VH antibody, immunoglobulin heavy constant mu antibody, Ighm antibody, ighm antibody, IGHM antibody

Background: AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region,IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker)
Cellular localisation: Cytoplasm, Cell Surface and Secreted

Molecular Weight: 50-75kDa

Gene ID: 3507, 510635

UniProt: P01871, P20769