CD103 antibody
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- Target See all CD103 (ITGAE) Antibodies
- CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD103 antibody is un-conjugated
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Application
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)
- Specificity
- This MAb recognizes a protein of 150 kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E beta-7 (aEb7) integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95 % of intraepithelial CD8+ cells and on 40 % of mucosa-associated T cells, whereas less than 2 % of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. Antibody to CD103 is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL).
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant human ITGAE protein fragment (exact sequence is proprietary)
- Clone
- ITGAE-2474
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product ITGAE Primary Antibody
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- Application Notes
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: Tonsil, Colon or Stomach.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))
- Alternative Name
- ITGAE (ITGAE Products)
- Synonyms
- A530055J10 antibody, CD103 antibody, aM290 antibody, alpha-E1 antibody, alpha-M290 antibody, HUMINAE antibody, integrin alpha E, epithelial-associated antibody, integrin subunit alpha E antibody, Itgae antibody, ITGAE antibody
- Background
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CD103, Human mucosal lymphocyte antigen 1 (HML1 antigen), Integrin alpha E heavy chain, Integrin alpha E light chain, Integrin alpha E, epithelial-associated, Integrin alpha E1, Integrin alpha-IEL (intestinal intraepithelial lymphocytes), Integrin alpha M290, Integrin alpha-E heavy chain, Integrin, alpha E (ITGAE),CD103 / Integrin alpha E (T-Cell Lymphoma & Hairy Cell Leukemia Marker)
Cellular localisation: Cell Surface and Cytoplasmic - Molecular Weight
- 150kDa
- Gene ID
- 3682, 513867
- UniProt
- P38570
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