Cytokeratin 13 antibody

Catalog No. ABIN6937655

Product Details anti-Cytokeratin 13 Antibody

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 13 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Cytokeratin 13 (KRT13) is the major acidic keratin, which together with KRT4, its basic partner, is expressed in the suprabasal layers of non-cornified stratified epithelia including tongue mucosa, esophagus, anal canal epithelium, tracheal epithelium, uterine cervix, and urothelium. Defects in the KRT13 gene are a cause of white sponge nevus of cannon (WSN), a rare autosomal dominant disorder, which predominantly affects non-cornified stratified squamous epithelia and is characterized by the presence of soft, white and spongy plaques in the oral mucosa. KRT13 has been used as a marker for non-keratinized squamous epithelium. It is also expressed in various squamous metaplasia, but it is down regulated in squamous dysplasia and squamous carcinoma.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human KRT13 protein

Clone: KRT13-2213

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (1-2 μg per million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or A431 cells. Skin or squamous cell carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Cytokeratin 13

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Alternative Name: KRT13 (KRT13 Products)

Synonyms: krt13 antibody, CK13 antibody, K13 antibody, Ka13 antibody, Krt-1.13 antibody, Krt1-13 antibody, ck13 antibody, k13 antibody, keratin 24 antibody, keratin 13 antibody, keratin 13, type I S homeolog antibody, krt24 antibody, KRT13 antibody, Krt13 antibody, krt13.S antibody, k13 antibody

Background: CK13, Cytokeratin-13, Keratin Type I Cytoskeletal 13, Keratin-13, KRT13, Type I Cytoskeletal 13, WSN2,Cytokeratin 13 (Non-Keratinized Squamous Epithelial Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 52kDa

Gene ID: 3860, 654550

UniProt: P13646