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Keratin 77 antibody

KRT77 Reactivity: Human IHC, WB, IF, FACS, IHC (f) Host: Mouse Monoclonal AE-1 unconjugated
Catalog No. ABIN6937737
  • Target See all Keratin 77 (KRT77) Antibodies
    Keratin 77 (KRT77)
    Reactivity
    • 25
    • 7
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 18
    • 7
    Mouse
    Clonality
    • 16
    • 9
    Monoclonal
    Conjugate
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Keratin 77 antibody is un-conjugated
    Application
    • 10
    • 9
    • 6
    • 5
    • 4
    • 3
    • 2
    Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificity
    This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
    Cross-Reactivity (Details)
    Human. Monkey. Cow. Dog. Rabbit. Mouse. Rat. Chicken. Turtle.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogen
    Human epidermal keratin
    Clone
    AE-1
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product KRT77 Primary Antibody
  • Application Notes

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Skin or squamous cell carcinoma (IHC).

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Preservative
    Azide free
    Storage
    -20 °C,-80 °C
    Storage Comment
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Expiry Date
    24 months
  • Target
    Keratin 77 (KRT77)
    Alternative Name
    KRT77 (KRT77 Products)
    Synonyms
    K1B antibody, KRT1B antibody, 4732484G22Rik antibody, K1emb antibody, Krt239 antibody, mK1b antibody, Kb39 antibody, keratin 77 antibody, KRT77 antibody, Krt77 antibody
    Background
    K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39,Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)
    Cellular localisation: Cytoplasmic
    Molecular Weight
    40-56.5kDa
    Gene ID
    374454, 334989
    UniProt
    Q7Z794
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