Recombinant Keratin 77 antibody

Catalog No. ABIN6937740

Product Details anti-Keratin 77 Antibody

Target: Keratin 77 (KRT77)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Keratin 77 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Cross-Reactivity (Details): Human. Dog. Expected to show broad species reactivity.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human KRT77 protein fragment

Clone: KRTL-1577R

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Skin, Squamous cell carcinoma (SCC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Keratin 77

Target: Keratin 77 (KRT77)

Alternative Name: KRT77 (KRT77 Products)

Synonyms: K1B antibody, KRT1B antibody, 4732484G22Rik antibody, K1emb antibody, Krt239 antibody, mK1b antibody, Kb39 antibody, keratin 77 antibody, KRT77 antibody, Krt77 antibody

Background: K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B,Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)

Gene ID: 374454, 334989

UniProt: Q7Z794