LGALS1/Galectin 1 antibody (AA 12-108)

Catalog No. ABIN6937774

Product Details anti-LGALS1/Galectin 1 Antibody

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Binding Specificity: AA 12-108

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LGALS1/Galectin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 Molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa12-108) of human Galectin-1 protein (exact sequence is proprietary)

Clone: GAL1-1831

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: JEG-3, HeLa, K562 or 293 cells. Prostate, Kidney, Placenta, Stomach, Skin, Spleen, Brain or Heart.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: LGALS1 (LGALS1 Products)

Synonyms: GAL1 antibody, GBP antibody, Galectin-1 antibody, LGAS1 antibody, LGALS1 antibody, MGC64502 antibody, GB10026 antibody, AA410090 antibody, Gal-1 antibody, Galbp antibody, L-14.5 antibody, L14 antibody, Lect14 antibody, galectin-1 antibody, Galaptin antibody, galectin 1 antibody, galectin-1 antibody, lectin, galactoside-binding, soluble, 1 L homeolog antibody, protein enabled antibody, lectin, galactose binding, soluble 1 antibody, LGALS1 antibody, Lgals1 antibody, CC1G_05003 antibody, CC1G_05004 antibody, lgals1.L antibody, LOC408848 antibody

Background: 14 kDa laminin-binding protein, Beta-galactoside-binding lectin L-14-I, Gal-1, GAL1, Galaptin, Galectin-1, HLBP14, HPL, Lactose-binding lectin 1, Lect14, Lectin galactoside-binding soluble 1, LGALS1, MAPK activating protein MP12, S-Lac lectin 1,Galectin-1 / Human Placental Lactogen (hPL)
Cellular localisation: Secreted (extracellular matrix)

Molecular Weight: 14kDa

Gene ID: 3956, 445351

UniProt: P09382

Pathways: Carbohydrate Homeostasis