MSH6 antibody (AA 374-540)

Catalog No. ABIN6937903

Product Details anti-MSH6 Antibody

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Binding Specificity: AA 374-540

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human MSH6 protein (around aa 374-540) (exact sequence is proprietary)

Clone: MSH6-3091

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HCT116, MCF-7 cells, Human colon carcinoma (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Alternative Name: MSH6 (MSH6 Products)

Synonyms: GTBP antibody, GTMBP antibody, HNPCC5 antibody, HSAP antibody, p160 antibody, cb326 antibody, wu:fb36c12 antibody, LOC100227810 antibody, AU044881 antibody, AW550279 antibody, Gtmbp antibody, mutS homolog 6 antibody, mutS homolog 6 (E. coli) antibody, DNA mismatch repair protein antibody, MSH6 antibody, msh6 antibody, Msh6 antibody

Background: DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, mutS (E. coli) homolog 6, MutS alpha 160 kDa subunit, MutS-alpha 160 kDa subunit, p160, Sperm associated protein,MSH6 (DNA Mismatch Repair Protein)
Cellular localisation: Nuclear

Molecular Weight: 163kDa

Gene ID: 2956, 445052

UniProt: P52701

Pathways: DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response