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Myc Tag antibody (AA 408-439)

Reactivity: Human IF, FACS, IHC (f) Host: Mouse Monoclonal 9E10-3 unconjugated

Catalog No. ABIN6937982

Datasheet as PDF

Target See all Myc Tag products

Myc Tag
Binding Specificity
32

21

12

8

8

3

2

1

1
AA 408-439
Reactivity
37

5

1
Human
Host
82

48

10

9

7

7

1

1
Mouse
Compatible Secondaries
Clonality
93

60

10
Monoclonal
Conjugate
80

14

12

8

4

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Myc Tag antibody is un-conjugated
Application
132

65

48

47

35

33

32

19

17

11

11

6

6

4

4

4

4

3

2

2

1

1

1

1

1
Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity

It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

No Cross-Reactivity

Chicken, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details)

Human. Does not react with Mouse, Rat or Chicken.

Purification

1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen

A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc, coupled to KLH.

Clone

9E10-3

Isotype

IgG1 kappa
anti-Myc Tag antibody
WB, IP, ELISA, IF Host: Mouse Monoclonal unconjugated

anti-Myc Tag (C-Term) antibody
Reactivity: Human WB, IP, IHC (p), FACS Host: Mouse Monoclonal 9E10 unconjugated

anti-Myc Tag antibody
WB, IP, ELISA Host: Rabbit Polyclonal unconjugated

anti-Myc Tag (C-Term) antibody (FITC)
Reactivity: Human FACS Host: Mouse Monoclonal 9E10 FITC

anti-Myc Tag (C-Term) antibody (Biotin)
Reactivity: Human WB, IP, IHC (p), FACS Host: Mouse Monoclonal 9E10 Biotin

anti-Myc Tag antibody
WB, IP, ELISA, IF Host: Mouse Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA Host: Mouse Monoclonal 9E10- unconjugated

anti-Myc Tag antibody
WB, IP, IF Host: Mouse Monoclonal 3B8 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal Myc-A7 unconjugated

anti-Myc Tag antibody (Biotin)
WB, IP, ELISA, IHC Host: Rabbit Polyclonal Biotin

Application Notes

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: HL-60 cells. Cervical Carcinoma.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative

Azide free

Storage

-20 °C,-80 °C

Storage Comment

Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date

24 months
Target

Myc Tag

Abstract

Myc Tag Products

Target Type

Tag

Background

Class E basic helix-loop-helix protein 39 (bHLHe39), MRTL, Myc2, Niard, Nird, Proto-oncogene c-Myc, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, V-Myc avian myelocytomatosis viral oncogene homolog,c-Myc Oncoprotein
Cellular localisation: Nuclear

Molecular Weight

62-64kDa

Gene ID

4609, 202453

UniProt

P01106