CD56 antibody

Catalog No. ABIN6938022

Product Details anti-CD56 Antibody

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD56 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135 kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Cross-Reactivity (Details): Human. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human NCAM1 protein

Clone: NCAM1-795

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Cerebellum, Pancreas, Neuroblastoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: NCAM1 (NCAM1 Products)

Synonyms: CD56 antibody, MSK39 antibody, NCAM antibody, fj56g09 antibody, ncam antibody, ncam1 antibody, wu:fj56g09 antibody, NCAM1 antibody, n-cam antibody, E-NCAM antibody, NCAM-1 antibody, Ncam antibody, Cd56 antibody, N-CAM antibody, N-CAM-1 antibody, NCAM-C antibody, NCAMC antibody, N-CAM-1-B antibody, NCAM-1-B antibody, ncam1-a antibody, ncam1-b antibody, neural cell adhesion molecule 1 antibody, neural cell adhesion molecule 1a antibody, neural cell adhesion molecule 1 S homeolog antibody, neural cell adhesion molecule 1 L homeolog antibody, NCAM1 antibody, ncam1a antibody, ncam1 antibody, LOC100085373 antibody, ncam1.S antibody, Ncam1 antibody, ncam1.L antibody

Background: NCAM, Leu-19, NKH1, MSK39, NCAM120, NCAM140, NCAM180, Neural Cell Adhesion Molecule,CD56 / NCAM1 / NKH1 (Neuronal Cell Marker)
Cellular localisation: Cell surface

Molecular Weight: 180, 145 and 125kDa

Gene ID: 4684, 503878

UniProt: P13591, P13592