14-3-3 sigma/SFN antibody
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- Target See all 14-3-3 sigma/SFN (SFN) Antibodies
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This 14-3-3 sigma/SFN antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- SFN (stratifin) is a p53-induced tumor suppressor gene which is activated in response to DNA damage, causing cell cycle arrest at G2 phase by blocking cdc2-cyclin B1 complex from entering the nucleus. It is inactivated in breast, lung, prostate, liver and gastric cancer. It is associated with poor prognosis when its down-regulation is observed in epithelial ovarian cancer. SFN expression could contribute to cancer cell proliferation and the development and/or progression of human gastrointestinal cancer.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant human full-length SFN protein
- Clone
- CPTC-SFN-2
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product SFN Primary Antibody
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- Application Notes
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Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: PC-3 cells, Skin and tissues enriched in stratified squamous keratinizing epithelium. HaCaT, hTCEpi, A-431 cell lines.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
- Alternative Name
- SFN (SFN Products)
- Synonyms
- YWHAS antibody, Er antibody, Mme1 antibody, Ywhas antibody, stratifin antibody, SFN antibody, Sfn antibody
- Background
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SFN, HME1, Stratifin, YWHAS, 14-3-3 Sigma,14-3-3 Sigma / Stratifin
Cellular localisation: Cytoplasm and nucleus. May be secreted by a non-classical secretory pathway. - Molecular Weight
- 28kDa
- Gene ID
- 2810, 523718
- UniProt
- P31947
- Pathways
- p53 Signaling, Myometrial Relaxation and Contraction
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