Transferrin Receptor antibody (AA 94-212)
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- Target See all Transferrin Receptor (TFRC) Antibodies
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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Binding Specificity
- AA 94-212
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Transferrin Receptor antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- It recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant extracellular fragment (around aa 94-212) of human TFRC protein (exact sequence is proprietary)
- Clone
- TFRC-1817
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product TFRC Primary Antibody
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- Application Notes
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat, HeLa, MCF-7 or K562 cells. Human placenta or Bone Marrow.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- Alternative Name
- TFRC (TFRC Products)
- Synonyms
- Trfr antibody, LOC100219280 antibody, tfr antibody, cd71 antibody, tfr1 antibody, trfr antibody, CD71 antibody, T9 antibody, TFR antibody, TFR1 antibody, TR antibody, TRFR antibody, p90 antibody, 2610028K12Rik antibody, AI195355 antibody, AI426448 antibody, AU015758 antibody, E430033M20Rik antibody, Mtvr-1 antibody, Mtvr1 antibody, transferrin receptor antibody, TFRC antibody, Tfrc antibody, tfrc antibody
- Background
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Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR,CD71 / Transferrin Receptor (TFRC) (Extracellular Domain)
Cellular localisation: Cell surface - Molecular Weight
- 85-95kDa (monomer), 190kDa (dimer)
- Gene ID
- 7037, 529618
- UniProt
- P02786
- Pathways
- Transition Metal Ion Homeostasis
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