TLE1 antibody (AA 175-338)

Catalog No. ABIN6938584

Product Details anti-TLE1 Antibody

Target: TLE1 (Transducin-Like Enhancer of Split 1 (E(sp1) Homolog, Drosophila) (TLE1))

Binding Specificity: AA 175-338

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein1 (TLE1) is a protein that isencoded by theTLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (aa 175-338) of human TLE1 (exact sequence is proprietary)

Clone: TLE1-2085

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, HepG2 or Jurkat cells. Endometrium Carcinoma or Sarcomas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TLE1

Target: TLE1 (Transducin-Like Enhancer of Split 1 (E(sp1) Homolog, Drosophila) (TLE1))

Alternative Name: TLE1 (TLE1 Products)

Synonyms: ESG antibody, ESG1 antibody, GRG1 antibody, C230057C06Rik antibody, Estm14 antibody, Grg1 antibody, Tle4l antibody, TLE1 antibody, transducin like enhancer of split 1 antibody, transducin-like enhancer protein 1 antibody, transducin-like enhancer of split 1 antibody, transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) antibody, TLE1 antibody, Smp_150080 antibody, Tle1 antibody, LOC100354911 antibody, LOC100524475 antibody, LOC100061341 antibody

Background: Enhancer of split groucho 1 (ESG1), Enhancer of split groucho-like protein 1, GRG1, TLE1, Transducin like enhancer of split 1, Transducin-like enhancer protein 1,TLE1 (Synovial Sarcoma Marker)
Cellular localisation: Nuclear

Molecular Weight: 83kDa

Gene ID: 7088, 197320, 689805

UniProt: Q04724

Pathways: WNT Signaling, SARS-CoV-2 Protein Interactome