CD137 antibody (AA 19-188)

Catalog No. ABIN6938625

Product Details anti-CD137 Antibody

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Binding Specificity: AA 19-188

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD137 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: CD137 belongs to the tumor necrosis factor receptor family and delivers a costimulatory signal to T lymphocytes. It is expressed on activated T cells and binds an inducible ligand that is found on B cells, macrophages and dendritic cells. Interactions between CD137 and its ligand are involved in antigen presentation and the generation of cytotoxic T cells. CD137 antibody may improve cancer treatment, and has been implicated in breast cancer, melanoma and lymphoma.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment (around aa 19-188) of human CD137 protein (exact sequence is proprietary)

Clone: 4-1BB-3201

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Immunofluorescence (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HEK293 cells, Jurkat whole cell lysate or recombinant human CD137 protein (WB). Human kidney cancer, liver, stomach or thyroid cancer tissue (IHC-P). Expressed on the surface of activated T-cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD137

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Alternative Name: TNFRSF9 (TNFRSF9 Products)

Synonyms: TNFRSF9 antibody, zgc:136557 antibody, 4-1BB antibody, CD137 antibody, CDw137 antibody, ILA antibody, A930040I11Rik antibody, AA408498 antibody, AI325004 antibody, Cd137 antibody, Ly63 antibody, tumor necrosis factor receptor superfamily member 9 antibody, TNF receptor superfamily member 9 antibody, tumor necrosis factor receptor superfamily, member 9a antibody, tumor necrosis factor receptor superfamily, member 9 antibody, TNFRSF9 antibody, tnfrsf9a antibody, Tnfrsf9 antibody

Background: 4-1BB Ligand Receptor T Cell, Antigen 4-1BB Homolog, CDw137, HLDA VI, Homolog of mouse 4 1BB, induced by lymphocyte activation (ILA), Interleukin activated receptor homolog of mouse Ly63, T-cell antigen 4-1BB / ILA homolog, Tumor necrosis factor receptor superfamily member 9,CD137 / 4-1BB / TNFRSF9
Cellular localisation: Cell Surface

Molecular Weight: 32kDa (monomer), 85kDa (dimer)

Gene ID: 3604

UniProt: Q07011

Pathways: Cancer Immune Checkpoints