p53 antibody (AA 14-389)

Catalog No. ABIN6938650

The Mouse Monoclonal anti-p53 antibody (Clone SPM349) (ABIN6938650) specifically detects p53 in WB, IF, FACS and IHC (fro). The antibody is reactive with Mouse samples.

Quick Overview for p53 antibody (AA 14-389) (ABIN6938650)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: SPM349

Product Details anti-p53 Antibody

Binding Specificity: AA 14-389

Specificity: The specificity of this monoclonal antibody to its intended target was tested by HuProtTM Array, containing more than 19,000, full-length human proteins. SPM349 binds to the C-terminus (aa213-217) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 Binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 Molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. p53 Is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70 % but incidence varies from zero in carcinoid lung tumors to 97 % in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.

Cross-Reactivity (Details): Human. Monkey. Cow. Dog. Mouse. Rat. Hamster.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Gel-Purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Frozen only) (1-2 μg/mL for 30 minutes at RT) Optimal dilution for a specific application should be determined.

Positive_Control: MDA-MB-231 or A431 cells. Breast or Colon carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: TP53

Background: Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53,p53 Tumor Suppressor Protein
Cellular localisation: Nuclear

Molecular Weight: 53kDa

Gene ID: 7157, 654481

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect