VEGFA antibody
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- Target See all VEGFA Antibodies
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This VEGFA antibody is un-conjugated
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Application
- Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
- Cross-Reactivity (Details)
- Human. Mouse. Rat. Rabbit. Dog.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Human VEGF189 recombinant protein
- Clone
- SPM225
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product VEGFA Primary Antibody
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- Application Notes
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Known_Application: Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (4-8 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA buffer, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: Tumor cells in astrocytomas, breast or ovarian carcinomas.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
- Alternative Name
- VEGFA (VEGFA Products)
- Synonyms
- MVCD1 antibody, VEGF antibody, VPF antibody, Vegf antibody, Vegf120 antibody, Vegf164 antibody, Vegf188 antibody, Vpf antibody, vegf antibody, vegfa antibody, wu:fj82c06 antibody, VEGF-A antibody, VEGF164 antibody, eVEGF120 antibody, eVEGF164 antibody, vegf-a antibody, vpf antibody, vefg antibody, si:dkey-14d8.3 antibody, wu:fd42e02 antibody, vascular endothelial growth factor A antibody, vascular endothelial growth factor Aa antibody, vascular endothelial growth factor A L homeolog antibody, vascular endothelial growth factor Ab antibody, VEGFA antibody, Vegfa antibody, vegfaa antibody, vegfa.L antibody, vegfa antibody, vegfab antibody
- Background
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MVCD1, Vascular Endothelial Growth Factor A (VEGF-A), Vascular Permeability Factor (VPF), VEGFA,VEGF (Vascular Endothelial Growth Factor)
Cellular localisation: Cytoplasmic, Cell Surface and Extracellular (Secreted) - Molecular Weight
- 19-22kDa (reducing) and 38kDa-44kDa (non-reducing)
- Gene ID
- 7422, 73793
- UniProt
- P15692
- Pathways
- RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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