Villin 1 antibody (AA 179-311)
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- Target See all Villin 1 (VIL1) Antibodies
- Villin 1 (VIL1)
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Binding Specificity
- AA 179-311
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Villin 1 antibody is un-conjugated
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Application
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- A recombinant fragment (around aa 179-311) of human Villin protein (exact sequence is proprietary)
- Clone
- VIL1-2376
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product VIL1 Primary Antibody
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- Application Notes
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: A549, HepG2 and HCT116 cells. Colon or Rectum.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- Villin 1 (VIL1)
- Alternative Name
- VIL1 (VIL1 Products)
- Synonyms
- D2S1471 antibody, VIL antibody, Vil antibody, vil1 antibody, MGC64339 antibody, VIL1 antibody, vil1l antibody, zgc:55779 antibody, villin 1 antibody, villin 1 S homeolog antibody, VIL1 antibody, Vil1 antibody, vil1.S antibody, vil1 antibody
- Background
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VIL1, Villin-1, Villin1,Villin (GI-Mucosal & Urogenital Brush Border Marker)
Cellular localisation: Cytoplasmic & Cell Surface - Molecular Weight
- 93kDa
- Gene ID
- 7429, 654595
- UniProt
- P09327
- Pathways
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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