N-Cadherin antibody (Cytoplasmic Domain)
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- Target See all N-Cadherin (CDH2) Antibodies
- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
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Binding Specificity
- Cytoplasmic Domain
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This N-Cadherin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
- Specificity
- Recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
- Cross-Reactivity (Details)
- Human and Mouse. Others not known.
- Purification
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
- Immunogen
- Recombinant human N-cadherin cytoplasmic domain
- Clone
- 13A9
- Isotype
- IgG1
- Top Product
- Discover our top product CDH2 Primary Antibody
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- Application Notes
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Positive Control: HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex
Known Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Western Blotting (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.
- Expiry Date
- 24 months
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- Target
- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
- Alternative Name
- CDH2 (CDH2 Products)
- Synonyms
- CD325 antibody, CDHN antibody, CDw325 antibody, NCAD antibody, N-cadherin antibody, cadherin-2 antibody, cdhn antibody, ncad antibody, cdw325 antibody, CDH2 antibody, Cadherin-2 antibody, Ncad antibody, glo antibody, lyr antibody, pac antibody, wu:fb47h04 antibody, cadherin 2 antibody, cadherin 2 S homeolog antibody, cadherin 2, type 1, N-cadherin (neuronal) antibody, CDH2 antibody, cdh2.S antibody, cdh2 antibody, Cdh2 antibody
- Background
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Cadherin-2 N cadherin neuronal, Cadherin-2 type 1, Cadherin-2, Calcium dependent adhesion protein neuronal, CD325, CDH2, CDHN, CDw325, N-Cadherin, NCAD,N-Cadherin / Cadherin-2 / CD325 (NCAD)
Cellular localisation: Cell surface - Molecular Weight
- 130-140kDa
- Gene ID
- 1000, 464829
- UniProt
- P19022
- Pathways
- Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane
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