FCGR3A antibody
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- Target See all FCGR3A Antibodies
- FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This FCGR3A antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)
- Specificity
- It recognizes CD16 (FcgammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. Two similar genes represent CD16, CD16A (FcgammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcgammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
- Purification
- Purified by Protein A/G
- Immunogen
- Human peripheral blood lymphocytes from a NK-leukemia patient.
- Clone
- HuNK2
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product FCGR3A Primary Antibody
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- Application Notes
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Positive Control: K-562 or U-937 cells. Lymph nodes and tonsils.
Known Application: Functional Studies (Order Ab without BSA & Azide), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
- Alternative Name
- FCGR3A (FCGR3A Products)
- Synonyms
- CD16 antibody, CD16A antibody, FCG3 antibody, FCGR3 antibody, FCGRIII antibody, FCR-10 antibody, FCRIII antibody, FCRIIIA antibody, IGFR3 antibody, Fcgr4 antibody, Fc fragment of IgG receptor IIIa antibody, FCGR3A antibody, Fcgr3a antibody
- Molecular Weight
- 50-80kDa
- Gene ID
- 2214
- UniProt
- P08637
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