HLA-DRB3 antibody

Catalog No. ABIN6939646

The Mouse Monoclonal anti-HLA-DRB3 antibody is suitable to detect HLA-DRB3 in samples from Human, Monkey, Baboon, Chimpanzee, Dog and Squirrel. It has been validated for IF and FACS.

Quick Overview for HLA-DRB3 antibody (ABIN6939646)

Target: HLA-DRB3 (Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3))

Reactivity: Human, Monkey, Baboon, Chimpanzee, Dog, Squirrel

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DRB3 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: Bra30

Product Details anti-HLA-DRB3 Antibody

Purpose: HLA-DRB (MHC II) Antibody

Specificity: This MAb reacts with the HLA-DRB3 antigen, a member of MHC class II molecules.It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human REH cells (non-T, non-B leukemia cell line)

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HLA-DRB3

Target: HLA-DRB3 (Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3))

Alternative Name: HLA-DRB3

Background:

HLA class II histocompatibility antigen DR beta 3 chain, HLADR3B, Major histocompatibility complex, class II DR beta 3

Daudi or HuT78 cells. Tonsil or lymph node., Ramos

Molecular Weight: ~28kDa (beta chain)

Gene ID: 3123, 534322

UniProt: P01911

Pathways: TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response